SCP0192

MMP 2 Substrate, fluorogenic

≥95% (HPLC), lyophilized

• Empirical Formula (Hill Notation): C₄₉H₆₈N₁₄O₁₅
• Molecular Weight: 1093.15
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• product name: MMP 2 Substrate, fluorogenic,
• Assay: ≥95% (HPLC)
• form: lyophilized
• composition: Peptide Content, ≥73%
• storage condition: protect from light
• storage temp.: −20°C

Description

Amino Acid Sequence

MCA-Pro-Leu-Ala-Nva-DNP-Dap-Ala-Arg-NH2

General description

Matrix metalloproteinases (MMPs) are localized to the nucleus, mitochondria, vesicles and cytoplasm. MMP family comprises of 25 members, of which 24 are present in mammals. MMPs are encoded as inactive enzymes and are localized to cell membranes. All MMP members possess pro domain (80 amino acids) and catalytic domains.

Application

Matrix metalloproteinases (MMP) are a family of zinc-dependent endopeptidases that degrade matrix proteins. MMPs include collagenases, gelatinases, matrilysins, enamelysins, metalloelastases, stromelysins and other structural protein and receptor lysins. The activity of these enzymes is selectively inhibited or measured by appropriately labeled peptides. MCA-Pro-Leu-Ala-Nva-DNP-Dap-Ala-Arg-NH2; DNP-Pro-Leu-Gly-Met-Trp-Ser-Arg (DNP-PLGMWSR), Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 (DNP-PLGLWA-D-R-NH2), Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH (DNP-PQGIAGQ-D-R-OH) and MOCAc [(7-methoxy coumarin-4-yl) acetyl]-RPKPYANvaWMK(Dnp[2,4-dinitrophenyl])-NH(2) are substrates for matrix metalloproteinase 2.

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) are responsible for degrading membrane proteins, such as basal lamina proteins of cerebral blood vessels. Proteolysis of intracellular substrates by MMPs is associated with the innate immune system and apoptosis. They are also involved in disease conditions such as oncogenesis, cardiac, neurological and autoimmune disorders. MMPs are also associated with pathogenesis related to protein conformational changes. Elevated expression of a number of MMPs is observed in myocardial infarction and heart failure.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable