R6878

Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-phospho-Rb (pSer⁷⁹⁵)
• MDL number: MFCD03457570
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RB-10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 110 kDa
• species reactivity: mouse, rat, human, bovine, hamster
• concentration: ~2 mg/mL
• technique(s): immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
• isotype: IgG1
• UniProt accession no.: P06400
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer795)
• Gene Information: human ... RB1(5925) ; mouse ... Rb1(19645) ; rat ... Rb1(24708)

Description

General description

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

Immunogen

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer⁷⁹⁵) of human retinoblastoma protein conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• fluorescence-activated cell sorting (FACS) assays
• enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS⁷⁹⁵ is required to inactivate Rb imposed growth suppression.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids