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GE28-9644-44

Sigma-Aldrich

HiScale 50/40

Synonym(s):

HiScale 50/40 Column, HiScale Column

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About This Item

UNSPSC Code:
41121800
NACRES:
SB.52

technique(s)

LPLC: suitable

Related Categories

General description

HiScale columns are delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors.

Features and Benefits

Adapter QuickLock mechanism, column measurement scale, and ergonomic design, facilitate column handling and increase process control.
Based on the well-established line of XK columns and compatible with all methods currently applicable to XK columns.
Capability to rinse entire adapter shaft after packing, thereby minimizing risk of bacterial growth after prolonged use.
Dual adapters, offering high flexibility and dynamic packing heights.
Empty columns with high pressure stability for packing with modern BioProcess media for process development and preparative chromatography.
Provides axial compression of the gel bed and a nonrotating plunger mechanism that expands packing capabilities.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a productā€™s label following the words ā€˜Lotā€™ or ā€˜Batchā€™.

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Articles

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efļ¬cient column packing and preparation for affinity chromatography of antibodies.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Performing a Purification of IgG Antibodies with Protein G SepharoseĀ® 4 Fast Flow

This page shows how to separate IgG antibodies by afļ¬nity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Puriļ¬cation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni SepharoseĀ® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efļ¬cient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation for Size Exclusion Chromatography

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