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Key Documents

RK-13 Cell Line from rabbit

21715, rabbit kidney, Epithelial-like

Synonym(s):

GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32190102

product name

RK-13 Cell Line from rabbit, rabbit kidney, 00021715

biological source

rabbit kidney

growth mode

Adherent

karyotype

2n=44, stem-line = 56 (=22%)

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rabbit kidney

Cell Line Description

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

Application

Virus studies

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

Other Notes

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Certificates of Analysis (COA)

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