This product is available as a 10cm x 10cm sheet. The membrane is also available in rolls of three lengths. Please see the link below to review these product options:
https://www.sigmaaldrich.com/product/compare?term=Immobilon%C2%AE-FL%20PVDF%20membrane&focus=products&page=1&perpage=30&sort=relevance&type=product&products=IPFL85R%3AMM%3Asial%2CIPFL00005%3AMM%3Asial%2CIPFL00010%3AMM%3Asial
05317
Immobilon®-FL PVDF membrane
pore size 0.45 μm
Synonym(s):
Low fluorescent blotting membranes
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About This Item
UNSPSC Code:
41105339
NACRES:
NB.22
Pricing and availability is not currently available.
Recommended Products
size
10 cm × 10 cm
pore size
0.45 μm
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Certificates of Analysis (COA)
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Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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Is the PVDF membrane offered in rolls or sheets?
1 answer-
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Can this PVDF membrane be used in Dot blot detecting nucleic acids?
1 answer-
No, a positively charged membrane is required for nucleic acid blotting. At this time, a positively charged PVDF membrane is not available. Nylon membranes are often used for this application. Please see the links below to review products that may be of interest:
Whatman Nytran N
https://www.sigmaaldrich.com/substance/whatmannytrannnylonblottingmembranes1234598765Roche Membrane
https://www.sigmaaldrich.com/product/roche/nylmroBlotting Nylon 66
https://www.sigmaaldrich.com/product/sial/15356Helpful?
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The membrane LF (low fluorescence) to Western blot ...... Is it made of PVDF ?
1 answer-
PVDF, as stated in the product name.
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