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MABN390

Sigma-Aldrich

Anti-Sigma IR Antibody, clone ICR-SIG1R-A

clone ICR-SIG1R-A, from rat

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ICR-SIG1R-A, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (immunogen homology)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2c

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SIGMAR1(10280)

General description

Sigma1R, or Sigma non-opioid intracellular receptor 1, is part of the ERG2 family that forms a ternary complex with ANK2 and ITPR3. Sigma1R is involved in lipid transport from the endoplasmic reticulum, BDNF and EGF signaling, potassium ion channel regulation and other cell functions including apoptosis, survival and proliferation. It was initially discovered through its binding to various psychoactive drugs, and has been studied in memory, learning and mood alteration processes. Sigma1R is often found in the nuclear membrane, synapses and endoplasmic reticulum of cells within liver, brain, kidney and thymus tissues.

Immunogen

Linear peptide corresponding to human Sigma IR.

Application

Detect the Sigma IR protein using this Anti-Sigma IR, clone ICR-SIG1R-A validated for use in wb, IP, ICC, Flow & IHC.
Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in certain cancer cell lysates.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in tumor cells.
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in colo-587 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sigma IR in 10 µg of colo-587 cell lysate.

Target description

~25 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~25 kDa, ~23 kDa, ~21.5 kDa, ~12 kDa and ~21 kDa

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Colo-587 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sohn G Kim et al.
Journal of leukocyte biology, 94(4), 835-843 (2013-07-03)
In vivo and in vitro exposure to stimulants has been associated with increased levels of HIV infection in PBMCs. Among these lymphocyte subsets, quiescent CD4(+) T cells make up the majority of circulating T cells in the blood. Others and

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