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MABF3179

Sigma-Aldrich

Anti-IL-6 Antibody, clone 3A7.10

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3A7.10, monoclonal

mol wt

calculated mol wt 23.72 kDa
observed mol wt ~21 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... IL6(3569)

General description

Interleukin-6 (UniProt: P05231; also known as IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2) is encoded by the IL6 (also known as IFNB2) gene (Gene ID: 3569) in human. IL-6 is an immunoregulatory secreted cytokine that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form (aa 30-212). It is mainly produced in skeletal muscle and its levels are shown to increase upon exercise due to enhanced production by contracting skeletal muscles. It is a potent inducer of the acute phase response and rapid IL-6 production contributes to host defense during infection and tissue injury. In the innate immune response, it is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection. In the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It is reported to plays a major role in the differentiation of CD4+ T cell subsets and is an essential factor for the development of T follicular helper (Tfh) cells. Its actions are mediated by binding to IL-6 receptor (IL-6R) and the ligand-receptor complex associates to the signaling subunit IL6ST/gp130. The interaction with the membrane-bound IL6R and IL6ST is reported to stimulate the classic signaling, whereas the binding of IL6 and soluble IL6R to IL6ST stimulates trans-signaling where synergistically with IL-1b and TNF it can induce the production of VEGF and stimulate angiogenesis. (Ref.: Nakahara, H., et al. (2003). Arthritis Rheum. 48(6); 1521-1529; Boulanger, MJ., et al. (2003). Science. 300(5628); 2101-2104).

Specificity

Clone 3A7.10 is a mouse monoclonal antibody that detects human IL-6.

Immunogen

Recombinant fragment of human Interleukin 6 (IL-6) corresponding to amino acids 29-212.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-6, clone 3A7.10, Cat. No. MABF3179, is a mouse monoclonal antibody that detects human Interleukin-6 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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