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EZRL

Millipore

Rat Leptin ELISA Kit

measures and quantifies Leptin levels in 10 μL plasma or serum

Synonym(s):

Leptin, Obesity Factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat Leptin ELISA, This Rat Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine research.

Quality Level

200

species reactivity

rat

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 102-122%
linearity: 87-100%
sensitivity: 0.04 ng/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

P41160
P50596

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Leptin is an adipokine secreted by the adipose tissue. It is also found in the primary and secondary lymphoid organs. Leptin has a 3D structure made up of four α-helix bundle motifs. It participates in regulating neuroendocrine function and energy homeostasis. Leptin also participates in physiological processes such as reproduction, angiogenesis, and hemopoiesis. Rat Leptin enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of leptin in rat sera. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user.

Application

Rat Leptin ELISA kit has been used in the determination of leptin from rat serum and plasma samples.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H317

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giuseppe Matarese et al.
Journal of immunology (Baltimore, Md. : 1950), 174(6), 3137-3142 (2005-03-08)
Leptin is an adipokine which conveys information on energy availability. In humans, leptin influences energy homeostasis and regulates neuroendocrine function primarily in states of energy deficiency. As a cytokine, leptin also affects thymic homeostasis and, similar to other proinflammatory cytokines
Staci D Bilbo et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(6), 2104-2115 (2010-02-04)
Obesity is well characterized as a systemic inflammatory condition, and is also associated with cognitive disruption, suggesting a link between the two. We assessed whether peripheral inflammation in maternal obesity may be transferred to the offspring brain, in particular, the
Effects of high-fat high-sucrose feeding, energy restriction, and trans-10,cis-12 conjugated linoleic acid on visfatin and apelin in hamsters.
Arrate Lasa,Itziar Churruca,Edurne Simon,Maria Teresa Macarulla et al.
Journal of the American College of Nutrition null
Low-frequency electro-acupuncture and physical exercise improve metabolic disturbances and modulate gene expression in adipose tissue in rats with dihydrotestosterone-induced polycystic ovary syndrome.
Louise Manner?s, Ingibjorg H Jonsdottir, Agneta Holmang, Malin Lonn, Elisabet Stener-Victorin
Endocrinology null
Bérengère Coupé et al.
PloS one, 7(1), e30616-e30616 (2012-02-01)
Intrauterine growth restriction (IUGR) is closely linked with metabolic diseases, appetite disorders and obesity at adulthood. Leptin, a major adipokine secreted by adipose tissue, circulates in direct proportion to body fat stores, enters the brain and regulates food intake and
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