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AB5895

Sigma-Aldrich

Anti-P2X3 Receptor Antibody, pain

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, primate

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... P2RX3(5024)

Specificity

P2X3

SPECIES REACTIVITIES:

Rat, mouse and primate. Other species have not been tested.

Immunogen

Synthetic peptide corresponding to the C-terminus of rat P2X3.

Application

Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB.
Immunoblotting: 1:500-1:1,000

Immunohistochemistry: 1:500-1:1,000

Immunocytochemistry: 1:500-1:1,000

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5895

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

IMMUNOCYTOCHEMISTRY

P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuroinflammation & Pain

Physical form

Rabbit serum. Liquid. Contains 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization.
Deiteren, A; van der Linden, L; de Wit, A; Ceuleers, H; Buckinx, R; Timmermans et al.
Testing null
The neurotrophin receptor p75 regulates gustatory axon branching and promotes innervation of the tongue during development.
Fei, D; Huang, T; Krimm, RF
Neural Dev. null
Tao Tang et al.
Scientific reports, 10(1), 22117-22117 (2020-12-19)
During development of the peripheral taste system, oral sensory neurons of the geniculate ganglion project via the chorda tympani nerve to innervate taste buds in fungiform papillae. Germline deletion of the p75 neurotrophin receptor causes dramatic axon guidance and branching
Tao Huang et al.
Developmental biology, 386(2), 308-320 (2014-01-01)
A limited number of growth factors are capable of regulating numerous developmental processes, but how they accomplish this is unclear. The gustatory system is ideal for examining this issue because the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) have
A subpopulation of nociceptors specifically linked to itch.
Han, L; Ma, C; Liu, Q; Weng, HJ; Cui, Y; Tang, Z; Kim, Y; Nie, H; Qu, L; Patel, KN; Li et al.
Nature Neuroscience null

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