Skip to Content
Merck
All Photos(1)

Key Documents

219330

Sigma-Aldrich

β-Catenin/Tcf Inhibitor, FH535

The β-Catenin/Tcf Inhibitor, FH535, also referenced under CAS 108409-83-2, controls the biological activity of β-Catenin/Tcf. This small molecule/inhibitor is primarily used for Cell Signaling applications.

Synonym(s):

β-Catenin/Tcf Inhibitor, FH535, N-(2-Methyl-4-nitro)-2,4-dichlorosulfonamide, Wnt Pathway Inhibitor IX

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C13H10Cl2N2O4S
CAS Number:
Molecular Weight:
361.20
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

Assay

≥99% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 100 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C13H10Cl2N2O4S/c1-8-6-10(17(18)19)3-5-12(8)16-22(20,21)13-7-9(14)2-4-11(13)15/h2-7,16H,1H3

InChI key

AXNUEXXEQGQWPA-UHFFFAOYSA-N

General description

A cell-permeable sulfonamide compound that acts as a dual pathway inhibitor against Wnt/β-catenin (≥80% inhibition at 15 µM in β-catenin/Tcf-dependent cellular reporter assays) and PPARγ/δ (≥30% inhibition at 0.5 µM in PPRE cellular reporter assays) signalings. Although both FH535 and the structurally similar PPARγ-selective inhibitor GW9662 (Cat. No. 370700) inhibit coactivator/PPAR binding, only FH535 blocks β-catenin/PPARγ interaction and the antagonistic activity of FH535 does not involve the covalent modification of or non-covalent interaction with the PPAR ligand-binding site cystein residue that is crucial for GW9662 action.
A cell-permeable sulfonamide compound that acts as a dual pathway inhibitor against Wnt/β-catenin (≥80% inhibition at 15 µM in β-catenin/Tcf-dependent cellular reporter assays) and PPARγ/δ (≥30% inhibition at 0.5 µM in PPRE cellular reporter assays) signalings. Although both FH535 and the structurally similar PPARγ-selective inhibitor GW9662 (Cat. No. 370700) inhibit coactivator/PPAR binding, only FH535 blocks β-catenin/PPARγ interaction and the antagonistic activity of FH535 does not involve the covalent modification of or non-covalent interaction with the PPAR ligand-binding site cystein residue that is crucial for GW9662 action.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Handeli, S., and Simon, J.A. 2008. Mol. Cancer Ther.7, 521.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Min Wang et al.
Journal of ovarian research, 17(1), 101-101 (2024-05-15)
Shikonin (SK), a naphthoquinone with anti-tumor effects, has been found to decrease production of tumor-associated exosomes (exo). This study aims to verify the treatment effect of SK on ovarian cancer (OC) cells, especially on the production of exo and their
Brandi Bailey et al.
Circulation research, 111(6), 750-760 (2012-07-18)
Cardiac progenitor cells are important for maintenance of myocardial structure and function, but molecular mechanisms governing these progenitor cells remain obscure and require elucidation to enhance regenerative therapeutic approaches. To understand consequences of stem cell antigen-1 (Sca-1) deletion on functional

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service