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06-661

Sigma-Aldrich

Anti-Calreticulin Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

pig, human, mouse, Xenopus, bovine, rat

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_004343

UniProt accession no.

P27797

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CALR(811)

Specificity

calreticulin; does not cross react with other endoplasmic reticulum proteins

Immunogen

peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin

Application

Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
Research Sub Category
PI3K, Akt, & mTOR Signaling

Enzymes & Biochemistry

Quality

routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts

Target description

60kDa

Linkage

Replaces: MABT145

Physical form

0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
Format: Purified

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria L Valencik et al.
Circulation research, 99(12), 1403-1410 (2006-11-11)
Integrins mechanically link the cytoskeleton to the extracellular matrix in cardiac myocytes and are thereby involved in mechanotransduction. Integrins appear to be necessary for cardiac myocyte hypertrophy. To determine the effect of increased integrin ligation and signaling on adult cardiac
M Waser et al.
The Journal of cell biology, 138(3), 547-557 (1997-08-11)
We have isolated and characterized a 12-kb mouse genomic DNA fragment containing the entire calreticulin gene and 2.14 kb of the promoter region. The mouse calreticulin gene consists of nine exons and eight introns, and it spans 4.2 kb of
R E Milner et al.
The Journal of biological chemistry, 266(11), 7155-7165 (1991-04-15)
The distribution of calsequestrin and calreticulin in smooth muscle and non-muscle tissues was investigated. Immunoblots of endoplasmic reticulum proteins probed with anti-calreticulin and anti-calsequestrin antibodies revealed that only calreticulin is present in the rat liver endoplasmic reticulum. Membrane fractions isolated
Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression.
Michalak, M, et al.
The Journal of Biological Chemistry, 271, 29436-29445 (1996)
Calreticulin inhibits glucocorticoid- but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultured McA-RH7777 hepatocytes.
Burns, K, et al.
Molecular and Cellular Biochemistry, 171, 37-43 (1997)
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