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Discovery® RP-Amide C16 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Agency

suitable for USP L60

Quality Level

feature

endcapped

manufacturer/tradename

Discovery®

extent of labeling

11% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

200 m2/g

surface coverage

2.6 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical base material

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Application

Discovery RP-AmideC16 HPLC Column may be used for the separation of:
  • Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
  • Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
  • Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
  • Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery® RP-AmideC16 HPLC column may be used for the separation of:
  • Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
  • Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
  • Hydrophilic bases by LC with principal component analysis (PCA).
  • 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Features and Benefits

  • Excellent retention and resolution of polar compounds
  • Unique selectivity compared to C18
  • Excellent peak shape and efficiency
  • Less hydrophobic than C18 columns
  • Compatible with 100% aqueous mobile phases
   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
HPLC separations of zidovudine and selected pharmaceuticals using a hexadecylsilane amide column.
Caufield WV and Stewart JT
Chromatographia, 54(9-10), 561-568 (2001)
Selection of reversed-phase liquid chromatographic columns with diverse selectivity towards the potential separation of impurities in drugs.
Van Gyseghem E, et al.
Journal of Chromatography A, 1042(1-2), 69-80 (2004)
Vitamin and fatty acid contents in seeds of some taxa belonging to genus Lathyrus L. growing in Turkey.
Sahin A, et al.
Acta Botanica Gallica, 156(3), 331-339 (2009)
Isomerization of aldehyde-2, 4-dinitrophenylhydrazone derivatives and validation of high-performance liquid chromatographic analysis
Uchiyama S, et al.
Journal of Chromatography A, 996(1-2), 95-102 (2003)

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