The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.
Specificity
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Application
Suitable for immunoblotting.
Physical form
Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Nature chemical biology, 12(4), 218-225 (2016-02-02)
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