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SRP3097

Sigma-Aldrich

sIL-6 Receptor alpha human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

B cell stimulatory factor-2, CD126, soluble IL-6 receptor alpha

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in CHO cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤5.0 ng/mL ED50

mol wt

37.9 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg endotoxin, tested

color

white to off-white

suitability

suitable for molecular biology

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL6R(3570)

General description

Interleukin-6 (IL-6) mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. The IL-6Rα chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6Rα. IL-6R has a role in various signaling cascades. The gp130 also can transmit signals from leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), interleukin-11 (IL-11) and cardiotrophin-1 (CT-1) in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6Rα which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6Rα is a 37.6kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
Research Area: IMMUNO AND CKS
Interleukin-6 Receptor Alpha (IL-6Rα) is a transmembrane protein.

Application

sIL-6 Receptor alpha human has been used in cell culture to treat fibroblasts. It has also been used in stimulation experiments to analyze IL-6 trans-signaling.

Biochem/physiol Actions

Interleukin-6 Receptor Alpha (IL-6Rα) plays a dual role in signaling. In classical signaling, it interacts with glycoprotein 130 to promote the anti-inflammatory and regenerative effects of IL-6. In contrast, during trans-signaling, it facilitates the pro-inflammatory effects of IL-6 by allowing cells lacking IL-6Rα to respond to IL-6 in the presence of soluble IL-6Rα. IL-6Rα exists in both soluble and transmembrane forms. The transmembrane form is integrated into the cell membrane of target cells. IL-6 and the IL-6 receptor alpha chain (IL-6R) are expressed by various cell types, including monocytes, lymphocytes, fibroblasts, and endothelial cells.

Sequence

LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD

Physical form

Lyophilized powder.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interleukin-6 signalling: more than Jaks and STATs
Eulenfeld R, et al.
European Journal of Cell Biology, 486-495 (2012)
Response to IL-6 trans-and IL-6 classic signalling is determined by the ratio of the IL-6 receptor alpha to gp130 expression: fusing experimental insights and dynamic modelling
Reeh H, et al.
Cell communication and signaling : CCS, 17, 1-21 (2019)
Hibi, M., et al.
Cytokine Reference, 1761-1773 (2001)
Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins
Corne TDJ, et al.
Cell adhesion & migration, 11(5-6), 447-463 (2017)
CNTF mediates neurotrophic factor secretion and fluid absorption in human retinal pigment epithelium.
Li R
PLoS ONE, 6(9), e23148-e23148 (2011)

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