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SAB4200692

Sigma-Aldrich

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

clone GST-2, purified from hybridoma cell culture

Synonym(s):

GST, Glutathione-S-Transferase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GST-2, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

ELISA: suitable
dot blot: suitable
immunoblotting: 0.5 μg/mL
immunofluorescence: suitable
immunoprecipitation (IP): suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.

Immunogen

purified recombinant GST fusion protein

Application

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • dot blotting
  • autoradiography
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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