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S6390

Sigma-Aldrich

Anti-Streptavidin antibody produced in rabbit

whole antiserum

Synonym(s):

Streptavidin Antibody, Streptavidin Antibody - Anti-Streptavidin antibody produced in rabbit

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About This Item

MDL number:
MFCD00162869
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

immunoelectrophoresis: suitable
indirect ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Streptavidin is a bacterial protein and a model system for studying protein interactions.

Immunogen

streptavidin from Streptomyces avidinii

Application

Anti-Streptavidin antibody produced in rabbit has been used in:
  • immunostaining
  • 3D Migration Assay
  • assessing phagosomal maturation
  • enzyme linked immunosorbent assay(ELISA)
  • immunoblotting

Biochem/physiol Actions

The streptavidin and its interaction with biotin is exploited in immunoassays and finds application in imaging, protein engineering, nanoassembly and cancer immunotherapy.

Analysis Note

This antisera is evaluated for performance and specificity by immunodiffusion and immunoelectrophoresis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Defined particle ligands trigger specific defense mechanisms of macrophages
Dykstra T, et al.
Innate Immunity, 17(4), 388-402 (2011)
How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer
Chivers CE, et al.
The Biochemical Journal, 435(1), 55-63 (2011)
Jason Wu et al.
Nature communications, 7, 12939-12939 (2016-10-04)
Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles
Interference from anti-streptavidin antibody
Rulander N, et al.
Archives of Pathology & Laboratory Medicine, 137(8), 1141-1146 (2013)
Cory M Alvey et al.
Current biology : CB, 27(14), 2065-2077 (2017-07-04)
Marrow-derived macrophages are highly phagocytic, but whether they can also traffic into solid tumors and engulf cancer cells is questionable, given the well-known limitations of tumor-associated macrophages (TAMs). Here, SIRPα on macrophages from mouse and human marrow was inhibited to
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