P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max^™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

• Synonym(s): PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO
• MDL number: MFCD00081839
• UNSPSC Code: 12161902
• PubChem Substance ID: 24898839
• NACRES: NA.75

Properties

• grade: Hybri-Max^™
• Quality Level: 200
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: solution
• mol wt: average mol wt 1,450
• concentration: 50 % (w/v)
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C
• SMILES string: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChI key: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Packaging

Packaged in sealed ampules under nitrogen.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable