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P7125
Pepsin from porcine gastric mucosa
powder, ≥400 units/mg protein
Synonym(s):
Pepsin A, Pepsin from hog stomach
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About This Item
Pricing and availability is not currently available.
Recommended Products
biological source
Porcine gastric mucosa
Quality Level
form
powder
specific activity
≥400 units/mg protein
mol wt
35 kDa
solubility
10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... LOC396892(396892)
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Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7125 is provided in powder form. Product P7125 has been used to denature DNA from kidney cells[1] and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining[2].
Biochem/physiol Actions
It does not cleave at valine, alanine, or glycine linkages.[5] Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.[5][6] Pepsin is inhibited by several phenylalanine-containing peptides.[7]
Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.[8]
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.[8]
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Unit Definition
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E1%/280
Other Notes
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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J Dahle et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(5), 1773-1778 (1997-03-04)
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Protocols
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
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Can you please tell me the Grade of this product?
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Hi, What is the shelf life for this product? Thanks
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Which Pepsin do you recommend for histology/antigen retrevial? According to protocol 0.2mg/ml in 0.2M HCL
1 answer-
This product has not been specifically qualified for antigen retrieval, however, it may be able to be qualified for use by the end user. A typical protocol using pepsin for antigen retrieval suggests the following stock and working solutions:
Pepsin Stock Solution (1% in 10mM HCl):
Pepsin ------------------------------------- 100 mg
10mM HCl (pH 2.0) ---------------------- 10 mlMix to dissolve. Store at -20 ºC
Pepsin Working Solution (0.5% in 5mM HCl):
Pepsin Stock Solution (0.5%) ------------ 1 ml
Distilled water ---------------------------- 1 mlMix well.
For antigen retrieval - cover sections with pepsin working solution and incubate for 10-20 minutes at 37 °C in humidified chamber (optimal incubation time may vary depending on tissue type and degree of fixation, and should be determined by user).
Product R2283 is a pepsin product qualified for antigen retrieval https://www.sigmaaldrich.com/product/sigma/r2283.
For more information, please see the following link: https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/immunohistochemistry/immunohistochemistry-protocol#antigen_retrieval.
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