MAK570

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 41116104

Properties

• Quality Level: 100
• absorbance ratio: _{570 nm}
• fluorescence: -535 nm-587 nm
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; cancer; neurological disorders
• storage temp.: −20°C

Description

General description

L(+)-Lactate emerges as a crucial metabolic byproduct within the animal body, synthesized via the enzyme Lactate Dehydrogenase (LDH). In the realm of cellular metabolism, lactate accrues as a result of vigorous cell division and under oxygen-deprived conditions, such as strenuous physical activity. Elevated lactate levels can signal various medical conditions, including oncological disorders, diabetes mellitus, and lactic acidosis. Notably, L(+)-Lactate, the predominant isomer generated during human metabolic processes, typically circulates in the bloodstream at concentrations ranging from 1 to 2 mmol/L.

Lactate plays a pivotal role in metabolism, particularly within the Cori cycle, where it serves as a link between anaerobic glycolysis in muscles and gluconeogenesis in the liver. During intense exercise when oxygen is scarce, pyruvate is converted into lactate by LDH, which can then be transported to the liver to synthesize glucose, thereby maintaining energy homeostasis. Additionally, lactate serves as an important energy source for the brain and heart, demonstrating its versatility beyond a mere end-product of anaerobic respiration.

Application

The Lactate Assay Kit may be used for:
Cancer Research
Metabolism Research
Neurological Research
Diabetes Research

Features and Benefits

Enhanced Sensitivity and Accuracy: Detect L-lactate levels ranging from 0.2 - 10 nmol/well providing precise results across a wide range of sample types.

Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.

Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file.

Suitability

Suitable for measuring L-Lactate in various biological samples such as cells, culture medium, and tissues.

Principle

In this assay, the quantification of lactate is achieved through an enzymatic method that yields a color change (observable at 570 nm) or a fluorescent signal (with an excitation wavelength of 535 nm and emission wavelength of 587 nm), both of which correlate directly with the amount of lactate in the sample. The sensitivity range of this kit is capable of detecting lactate concentrations as low as 0.2 - 10 nmol/well.

Kit Components Only

• Assay Buffer
• Lactate Probe in DMSO
• Lactate Enzyme Mix
• L-Lactate Standard

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334,H412
• Precautionary Statements: P261 - P273 - P284 - P501
• Hazard Classifications: Aquatic Chronic 3 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2