Yes, this kit quantifies total collagen but does not distinguish between specific types.
MAK322
Collagen Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Collagen Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 fluorometric tests
input
cell
serum
plasma
solution(s) (collagen)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
rheumatological diseases; dermatological diseases; gastrointestinal diseases; pulmonary disorders; cancer
storage temp.
−20°C
General description
Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.
Application
The Collagen Assay Kit may be used for:
- Bone Disorder Research
- Dermatological Research
- Wound Healing and Tissue Repair Research
Features and Benefits
Enhanced Detection Range: Detect Collagen levels accurately across a wide range, from 2 µg/mL to 50 µg/mL, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
Suitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)
Principle
Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B - Resp. Sens. 1
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
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Does this kit detect total collagen?
1 answer-
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Will the assay work if the samples to be dosed are either or both pH’s, and do they have to be neutralized? If so, to what pH (from both extremes)? Also, Does the collagen standard also have to be blanked?
1 answer-
1. The samples should be neutralized before assaying. The assay buffer is around pH 7.5, so it is recommended that samples be neutralized to around pH 7 to 8.
2. The collagen standard does not have to be blanked. During calculation, standard 4 (only water) is subtracted from the other standards as a blank.Helpful?
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Would the MAK322 Collagen Assay Kit be suitable for use with whole cell lysates containing RIPA buffer?
1 answer-
No tests have been conducted on the effect of RIPA on this assay. It is believe that RIPA-treated cell lysates should be compatible with the kit, as long as they do not contain chelation agents such as EDTA. However, they discourage the use of chelators because the collagenase used in the assay requires calcium as a cofactor.
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Is this kit compatible with cell lysates in detergent buffers (ex. RIPA buffer)?
1 answer-
The compatibility of RIPA in this assay has not been evaluated. However, no interference is anticipated. The use of any additional chelating agents, such as EDTA, are not recommended and will negatively impact results.
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