MAK223
Aldolase Activity Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Aldolase Colorimetric Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
relevant disease(s)
cancer; neurological disorders; hematological disorder
storage temp.
−20°C
Gene Information
human ... ALDOA(226) , ALDOB(229) , ALDOC(230)
mouse ... ALDOA(11674) , ALDOB(230163) , ALDOC(11676)
rat ... ALDOA(24189) , ALDOB(24190) , ALDOC(24191)
General description
Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development. Aldolases have been recognized as positive regulators of the Wnt signaling pathway. Deficiency of aldolases is associated with myopathy and hemolytic anemia.
The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.
The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.
Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development. Aldolases have been recognized as positive regulators of the Wnt signaling pathway. Deficiency of aldolases is associated with myopathy and hemolytic anemia
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of Aldolase activity in biological samples such as tissue, cells, serum, and plasma.
Principle
The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.
Kit Components Only
Product No.
Description
- Aldolase Assay Buffer
- Aldolase Substrate
- Aldolase Enzyme Mix
- Aldolase Developer
- NADH Standard
- Aldolase Positive Control
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Aldolase positively regulates of the canonical Wnt signaling pathway.
Caspi M, et al.
Molecular Cancer, 13(1), 164-164 (2014)
Human aldolase A natural mutants: relationship between flexibility of the C-terminal region and enzyme function.
Esposito G, et al.
The Biochemical Journal, 380(1), 51-56 (2004)
Zhenchang Guo et al.
Molecular & cellular proteomics : MCP, 20, 100053-100053 (2021-02-10)
Esophageal squamous cell carcinoma (ESCC) is an aggressive malignancy with poor therapeutic outcomes. However, the alterations in proteins and posttranslational modifications (PTMs) leading to the pathogenesis of ESCC remain unclear. Here, we provide the comprehensive characterization of the proteome, phosphorylome
Unraveling the structural and functional features of an aldolase A mutant involved in the hemolytic anemia and severe rhabdomyolysis reported in a child.
Esposito G, et al.
Blood, 105(2), 905-906 (2005)
Biochemical markers of muscular damage.
Brancaccio P, et al
Clinical Chemistry and Laboratory Medicine, 48(6), 757-767 (2010)
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