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MAK088

Sigma-Aldrich

DPP4 Activity Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

CD26 Activity Assay Kit, Deaminase Complexing Protein 2 Activity Assay Kit, Dipeptidyl Peptidase-4 Activity Assay Kit

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)

General description

Dipeptidyl peptidase-4 (DPP4), also known as CD26 and adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-Proline and X-Alanine residues from the N-termini of polypeptides.[1] DPP4 is a transmembrane glycoprotein whose activity regulates the bioactivity of multiple peptides such as growth factors, chemokines, and neuropeptides. DPP4 plays a major role in glucose metabolism via the regulation of glucagon-like peptide-1 and inhibitors of DPP4 are commonly used for the treatment of type 2 diabetes. DPP4 also plays an important role in immune regulation and may play a role in tumor suppression. Deletion of the DPP4 gene affects carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of the protein is observed during liver regeneration, cirrhosis and liver tumorigenesis.[2][3]

Application

DPP4 Activity Assay Kit has been used to determine the activity of dipeptidyl peptidase-4 (DPP4) in homogenates.[4][5]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of dipeptidyl peptidase-4 (DPP4) in a variety of samples including tissue, cells and serum

Principle

The DPP4 Activity Assay kit provides a simple and direct procedure for measuring DPP4 activity in a variety of samples. In this assay, DPP4 cleaves a non-fluorescent substrate, H-Gly-Pro-AMC, to release a fluorescent product, 7-Amino-4-Methyl Coumarin (AMC) (λex = 360/λem = 460 nm). One unit of DPP4 is the amount of enzyme that will hydrolyze the DPP4 substrate to yield 1.0 μmole of AMC per minute at 37 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
8E03K07790
8E18K07790
8G29K07790
6M29K07790
6L06K07790

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DPP-IV Inhibitory Potential of Methanolic Extract of Pueraria Tuberosa in Liver of Alloxan Induced Diabetic Model.
Srivastava S, et al.
Biosciences, Biotechnology Research Asia, 15(1), 01-04 (2018)
Xinyu Wang et al.
Frontiers in pharmacology, 12, 779652-779652 (2021-12-25)
Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease and has become a serious public health problem worldwide. Dipeptidyl peptidase-4 (DPP4) inhibitors, an emerging drug for the treatment of diabetes, have been found to have renoprotective effects
Dipeptidyl peptidase-4 is highly expressed in bronchial epithelial cells of untreated asthma and it increases cell proliferation along with fibronectin production in airway constitutive cells.
Shiobara T, et al.
Respiratory Research, 17(1), 28-28 (2016)
Yashini Govender et al.
Clinical immunology (Orlando, Fla.), 230, 108824-108824 (2021-08-16)
The current intersection of the COVID-19 and HIV-1 pandemics, has raised concerns about the risk for poor COVID-19 outcomes particularly in regions like sub-Saharan Africa, disproportionally affected by HIV. DPP4/CD26 has been suggested to be a potential therapeutic target and
Yi-Chen Li et al.
Communications biology, 4(1), 144-144 (2021-01-31)
Peritoneal dialysis (PD) possesses multiple advantages for end stage renal disease. However, long-term PD triggers peritoneal fibrosis (PF). From the nationwide analysis of diabetic PD patients (n = 19,828), we identified the incidence of PD failure was significantly lower in diabetic patients
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Questions

1–3 of 3 Questions  

  1. Which is th origin of the DPP4 positive control included in the kit, is it human or porcine?

    1 answer

    1. The positive control in this kit is a recombinant human DPP4.

      Helpful?

  2. Hi, I purchased DPP4 Kit (MAK088) for testing pulse flour samples. The DPP4 assay buffer is limited in amount for hydrating pulse flour samples. Could I use 0.1M, pH8 Tris-HCl buffer instead? and what is the major component of your DPP4 assay buffer?

    1 answer

    1. The assay buffer supplied in Kit MAK088 is optimized for the reactions they are designed for. It is recommended to use the buffers in the kit. The components within the buffer are considered confidential and cannot be disclosed.

      Helpful?

  3. Pourrait-on savoir les composants de ce MAK088-1KIT s'il vous plait?

    1 answer

    1. The DPP4 Activity Assay Kit contains:
      DPP4 Assay Buffer 25 mL
      DPP4 Substrate, H-Gly-Pro-AMC 0.2 mL
      DPP4 Positive Control 20 mL
      AMC Standard, 1 mM 0.1 mL
      DPP4 Inhibitor, Sitagliptin 1 mL

      Helpful?

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