L5520

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Leibovitz L15
• MDL number: MFCD00217482
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: 0.011 g/L; sodium pyruvate: 0.55 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO₂) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  

• in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
• as a supplement in sterile cell media for the dissection and isolation of kidney cells
• as a component of culture buffer for the tissue culture of Newt lung cells

Reconstitution

Supplement with 0.3 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable