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L1516

Sigma-Aldrich

Lectin from Wisteria floribunda

buffered aqueous solution, biotin conjugate

Synonym(s):

Wisteria floribunda agglutinin, WFA

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About This Item

MDL number:
MFCD00166187
UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

200

form

buffered aqueous solution

packaging

pkg of 2 mg active conjugate

storage temp.

2-8°C

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Biochem/physiol Actions

WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

Packaging

Package size based on active conjugate content

Preparation Note

Protein is biotinylated utilizing aminocaproyl spacer.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elise Holter Thompson et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(3), 607-612 (2017-12-28)
Throughout life animals learn to recognize cues that signal danger and instantaneously initiate an adequate threat response. Memories of such associations may last a lifetime and far outlast the intracellular molecules currently found to be important for memory processing. The
Soledad Domínguez et al.
Cell reports, 29(5), 1099-1112 (2019-10-31)
Adolescence is a vulnerable period characterized by major cognitive changes. The mechanisms underlying the emergence of new cognitive functions are poorly understood. We find that a long-term depression of inhibitory transmission (iLTD) from parvalbumin-expressing (PV+) interneurons in the hippocampal area Cornu
Vanessa Cattaud et al.
Neurobiology of aging, 72, 147-158 (2018-10-03)
Recent findings show that parvalbumin (PV) interneuron function is impaired in Alzheimer's disease (AD), and that this impairment in PV function can be linked to network dysfunction and memory deficits. PV cells are often associated with a specific extracellular matrix
Kelly E Carstens et al.
The European journal of neuroscience, 53(12), 4005-4015 (2020-11-22)
Current methods of experimentally degrading the specialized extracellular matrix (ECM), perineuronal nets (PNNs) have several limitations. Genetic knockout of ECM components typically has only partial effects on PNNs, and knockout of the major ECM component aggrecan is lethal in mice.
Carly M Drzewiecki et al.
Brain structure & function, 225(8), 2495-2507 (2020-09-12)
The prefrontal cortex (PFC) is a late developing region of the cortex, and its protracted maturation during adolescence may confer a period of plasticity. Closure of critical, or sensitive, periods in sensory cortices coincides with perineuronal net (PNN) expression, leading
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