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H7165

Sigma-Aldrich

Anti-HtrA2 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase-2, Anti-OMI, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~36 kDa

species reactivity

rat, human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 2-4 μg/mL using rat liver microsomal fraction

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HTRA2(27429)
mouse ... Htra2(64704)
rat ... Htra2(297376)

General description

High temperature requirement protein A2 (HtrA2) or serine peptidase 2 belongs to the HtrA family of serine proteases. It is a mitochondrial serine protease, that comprises of an N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM) and a single C-terminal PDZ domain. It also has a transmembrane and a serine protease domain. HTRA2 gene is mapped to human chromosome 2p13.1.

Specificity

Anti-HtrA2 (C-terminal) specifically recognizes human and rat HtrA2.

Application

Anti-HtrA2 (C-terminal) antibody produced in rabbit may been used in immunoblotting.

Biochem/physiol Actions

High temperature requirement protein A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. It is essential for mitochondrial homeostasis and regulates apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2, via its IAP binding domain favors the neutralizing inhibitor of apoptosis proteins (IAPs) by promoting the cytochrome c-dependent caspase activation. Mutations that inactivate HtrA2 gene are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease. Mutation in the HtrA2 gene is also implicated in the early-onset mitochondrial syndromes including the 3-methylglutaconic aciduria. HtrA2 variants are responsible infantile neurodegeneration, mitochondrial dysfunction and promote apoptosis.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L Vande Walle et al.
Cell death and differentiation, 15(3), 453-460 (2008-01-05)
The HtrA family refers to a group of related oligomeric serine proteases that combine a trypsin-like protease domain with at least one PDZ interaction domain. Mammals encode four HtrA proteases, named HtrA1-4. The protease activity of the HtrA member HtrA2/Omi
Dorota Zurawa-Janicka et al.
Expert opinion on therapeutic targets, 14(7), 665-679 (2010-05-18)
The HtrA family proteins are serine proteases that are involved in important physiological processes, including maintenance of mitochondrial homeostasis, apoptosis and cell signaling. They are involved in the development and progression of several pathological processes such as cancer, neurodegenerative disorders
Hyo-Jin Park et al.
The Journal of biological chemistry, 281(45), 34277-34287 (2006-09-14)
The processing and metabolism of amyloid precursor protein (APP) is a major interest in Alzheimer disease (AD) research, because not only amyloid beta (Abeta) peptide, but also cellular or mitochondrial APP are intimately involved in cellular dysfunction and AD pathogenesis.
Monika Oláhová et al.
Journal of inherited metabolic disease, 40(1), 121-130 (2016-10-04)
Mitochondrial diseases collectively represent one of the most heterogeneous group of metabolic disorders. Symptoms can manifest at any age, presenting with isolated or multiple-organ involvement. Advances in next-generation sequencing strategies have greatly enhanced the diagnosis of patients with mitochondrial disease

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