Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

E9030

Sigma-Aldrich

Endoglycoceramidase II from Rhodococcus sp.

aqueous solution

Synonym(s):

EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
3.2.1.123 (BRENDA, IUBMB)
MDL number:
MFCD03456712
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

conjugate

(Lipid-linked)

form

solution

mol wt

58.9 kDa

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.

Unit Definition

One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.

Physical form

Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A E Manzi
Current protocols in molecular biology, Chapter 17, Unit17-Unit17 (2008-02-12)
Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used
Assays for transmembrane movement of sphingolipids.
D J Sillence et al.
Methods in enzymology, 312, 562-579 (2000-11-09)
Xu Xu et al.
Journal of biochemistry, 141(1), 1-7 (2006-12-15)
The use of bovine brain has been prohibited in many countries because of the world-wide prevalence of mad cow disease, and thus porcine brain is expected to be a new source for the preparation of gangliosides. Here, we report the
The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system.
Matthew E C Caines et al.
Angewandte Chemie (International ed. in English), 46(24), 4474-4476 (2007-05-10)
Yohei Ishibashi et al.
Glycobiology, 19(7), 797-807 (2009-04-25)
Although 6-gala series glycosphingolipids possessing R-Gal (alpha/beta) 1-6Gal beta 1-1'Cer have been found in some mollusks, pathogenic parasites, and fungi, their physiological functions and metabolic pathway are not fully understood. We described a novel method of detecting 6-gala series glyco-
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service