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D9768

Sigma-Aldrich

Lambda Phage DNA, Methylated from Escherichia coli host strain W3110

lyophilized powder

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
NACRES:
NA.52

grade

for molecular biology

Quality Level

form

lyophilized powder

mol wt

31.5 × 103 kDa
48 kb

suitability

suitable for substrate for restriction endonucleases

storage temp.

2-8°C

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General description

Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This methylated lambda DNA is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I.

Specificity

Unique restriction sites: Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I. Methylated lambda is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I.

Application

Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 has been used in DNA combing to determine the conversion factor.
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used for immunoprecipitation in ChIP assays.
The lambda phage has an icosahedral head and a long tail terminating in a single fiber. At both ends of the 5′ termini are complementary 12-nucleotide single strand sequences that contribute to the cohesive ends (cos region) of the DNA. The tail of the phage latches on the host outer membrane receptor and injects phage DNA into the cell. The phage converts the E. coli to a lysogenic state in which the phage functions are repressed and the phage genome may remain dormant (prophage) for a long time. This property is seen in bacteriophages that carry CII and CIII genes that are responsible for CI expression. Bacteriophages with CI mutation in the CI gene are able to maintain a lysogenic state at defined temperatures.
Infecting E. coli strain W3110 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.

Unit Definition

One O.D. (A260) is approximately 50 μg of DNA

Reconstitution

Add the appropriate amount of 1x Tris-EDTA buffer, pH 8.0 (Product No. T9285, diluted to 1x, or comparable), to the vial.
Incubate the DNA sample vial at 65 °C for 4 hrs with frequent mixing.
After reconstitution, the product should be stored at -20 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of replicating yeast chromosomes by DNA combing
Gallo D,et al.
Cold Spring Harbor Protocols, 2016(2), pdb-prot085118 (2016)
P Tran et al.
Physical review letters, 85(7), 1564-1567 (2000-09-06)
We have measured the conductivity sigma along the lambda phage DNA (lambda-DNA) double helix at microwave frequencies using lyophilized DNA in and also without a buffer. The conductivity is strongly temperature dependent around room temperature with a crossover to a
Alec M Desimone et al.
Molecular and cellular biology, 30(13), 3342-3356 (2010-05-05)
Switching between alternate states of gene transcription is fundamental to a multitude of cellular regulatory pathways, including those that govern differentiation. In spite of the progress in our understanding of such transitions in gene activity, a major unanswered question is

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