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Key Documents

OE33 Cell Line human

96070808, human esophagus, Epithelial

Synonym(s):

JROECL33, JROECL33 Cells, OE-33 Cells

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About This Item

UNSPSC Code:
41106514

product name

OE33 Cell Line human, 96070808, Caucasian esophageal carcinoma

biological source

human esophagus

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial

products

Not specified

receptors

MHC class I, ICAM-1

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian oesophageal carcinoma

Cell Line Description

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

Application

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.
Study of oesophageal cancer

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

Other Notes

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Certificates of Analysis (COA)

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