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A4781

Sigma-Aldrich

Asialofetuin from fetal calf serum

Type I (Sigma designation)

Synonym(s):

Asialofetuin

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

bovine (calf) serum

Quality Level

type

Type I (Sigma designation)

form

powder

impurities

salt, essentially free
≤0.5% N-acetylneuraminic acid

solubility

0.85% sodium chloride: soluble 1 mg/mL

storage temp.

2-8°C

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General description

Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.

Application

Asialofetuin from fetal calf serum has been used:
  • to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
  • quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
  • as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
  • to study the nature of the interaction between ferritin and the placenta
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.

Preparation Note

Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Jakob Meier-Credo et al.
Journal of the American Society for Mass Spectrometry, 33(7), 1293-1302 (2022-06-28)
Identification and sequence determination by mass spectrometry have become routine analyses for soluble proteins. Membrane proteins, however, remain challenging targets due to their hydrophobicity and poor annotation. In particular small membrane proteins often remain unnoticed as they are largely inaccessible
Nida Zaidi et al.
International journal of biological macromolecules, 103, 65-73 (2017-05-13)
The SDS-glycoprotein system is mimic of membrane protein-lipid system. Fate of glycoprotein, conformation and the interactive forces involved in membrane milieu are expected to be decided by the net charge on glycoprotein that may change during acidic environment in a
Keiichi Motoyama et al.
Journal of drug delivery, 2011, 476137-476137 (2011-04-15)
The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed
R D Lamparelli et al.
British journal of haematology, 72(1), 100-105 (1989-05-01)
The organ distribution of intravenously injected hepatic ferritin either labelled with 59Fe or with 59Fe and 125I, was studied in pregnant guinea-pigs. At 5 h 71.2% of injected 59Fe was present in the placenta and fetus. Transfer of 59Fe to
Antra Ganguly et al.
Current protocols, 1(6), e150-e150 (2021-06-09)
Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions. Subtle changes in glycans resulting from altered glycosylation

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