Skip to Content
Merck
All Photos(1)

Documents

A3437

Sigma-Aldrich

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Human IgM (μ-chain specific)−AP

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. These immunoglobulins have been implicated in CNS myelin repair. Increased levels of secretory IgM have been linked to rhino-conjunctivitis . Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

Immunogen

Human IgM

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA.
Goat Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody can be used for dot blot and western blot applications at 1:30,000.
Goat polyclonal anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques.
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

F Petry
Infection, 26(1), 7-10 (1998-03-20)
In a seroprevalence study including 495 sera from persons of all age-groups, the presence of anti-Cryptosporidium parvum antibodies was evaluated in an enzyme immunoassay. Despite the fact that C. parvum is only found in approximately 2% of patients with diarrhea
Helena Cerutti et al.
Journal of clinical laboratory analysis, 35(4), e23735-e23735 (2021-02-21)
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for
Melissa D Klein et al.
PLOS global public health, 2(7), e0000619-e0000619 (2023-03-25)
Meat packing, produce processing, and farm workers are known to have an elevated risk of COVID-19, but occupational risk factors in this population are unclear. We performed an observational cohort study of meat packing, produce processing, and farm workers in
Chunsheng Jin et al.
The Journal of biological chemistry, 298(4), 101732-101732 (2022-02-18)
Changes in glycosphingolipid structures have been shown to occur during the development of several types of human cancers, generating cancer-specific carbohydrate structures that could be used as biomarkers for diagnosis and therapeutic targeting. In this study, we characterized nonacid glycosphingolipids
S Ghosh et al.
Methods in molecular medicine, 41, 257-277 (2000-01-01)
Studies of intestinal mucosal immunity and inflammation are limited by the relative inaccessibility of most of the small intestine. Any new method of studying mucosal immunity and inflammation in patients should be minimally invasive, cost-effective, and provide information not readily

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service