A2765

Azocasein

protease substrate, chromogenic, powder

• Synonym(s): Sulfanilamide-azocasein
• CAS Number: 102110-74-7
• MDL number: MFCD00130592
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• product name: Azocasein, protease substrate
• biological source: bovine
• Quality Level: 200
• form: powder
• technique(s): ligand binding assay: suitable
• solubility: water: 5 mg/mL, clear, orange to very deep orange
• ε (extinction coefficient): ≥25 at 440 nm in 0.1 M NaOH at 1%
• storage temp.: 2-8°C

Description

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves