Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

F6377

Sigma-Aldrich

Fluorescein sodium salt

Fluorescein sodium salt

Powder

Synonym(s):

Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
518-47-8
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein:
3833041
EC Number:
208-253-0
MDL number:
MFCD00167039
UNSPSC Code:
12171500
PubChem Substance ID:
24894907
NACRES:
NA.47
Pricing and availability is not currently available.

Product Name

Fluorescein sodium salt, used as fluorescent tracer

Quality Level

200

form

powder

technique(s)

titration: suitable

color

orange to brown

pH

8.3 (20 °C, 10 g/L)

mp

>300 °C

solubility

H2O: 1 mg/mL

density

0.78 g/cm3 at 29.6 °C (85.3 °F at 699.7 hPa)

fluorescence

λex 460 nm; λem 515 nm(lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Fluorescein is a xanthene dye and is a common labeling agent in biology.[1]

Application

Fluorescein sodium salt has been used as a solute to study microvessel permeability.[2][3] It has been used as a test molecule to study blood barrier penetration.[4]
Water soluble fluorescent dye used in permeability and vascular perfusion studies due to its small molecular radius.[5] Sodium fluorescein is used as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models.[6][7][8] The dye was used as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes.[9] Sodium fluorescein was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP).[10]

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

423.7 °F - Pensky-Martens closed cup

Flash Point(C)

217.6 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBS2708
SHBS3018
0000240645
0000150889
0000182362

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tom De Bruyn et al.
Journal of pharmaceutical sciences, 100(11), 5018-5030 (2011-08-13)
The aim of this study was to characterize the in vitro hepatic uptake kinetics of sodium fluorescein (NaFluo) and identify the transporters involved. NaFluo exhibited saturable uptake kinetics in suspended rat and human hepatocytes as reflected by K(m) values of
Electrical conductivity and dielectric relaxation behavior of fluorescein sodium salt (FSS).
Mansour SA, et al.
Solid State Communications, 150(29), 1386-1391 (2010)
R Oishi et al.
Naunyn-Schmiedeberg's archives of pharmacology, 339(1-2), 159-165 (1989-01-01)
Morphine (5 mg/kg, s.c.) caused a submaximal increase in the brain level of sodium fluorescein administered i.v. Histamine H1-antagonists, diphenhydramine and mepyramine, given either i.p. or i.c.v., had no significant influence on the effect of morphine. H2-Antagonists, cimetidine and ranitidine
Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design (2010)
Dimitris N Xanthos et al.
Molecular pain, 8, 44-44 (2012-06-21)
Changes in the blood-central nervous system barriers occur under pathological conditions including inflammation and contribute to central manifestations of various diseases. After short-lasting peripheral and neurogenic inflammation, the evidence is mixed whether there are consistent blood-spinal cord changes. In the
View All Related Papers
1 Review This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. What is the purity of Fluorescein sodium salt (CAS number 518-47-8, article number F6377-100G)?

    1 answer

    1. This purity of this product is not determined.

      Helpful?

  2. Can this product be autoclaved once dissolved? How long can a concentrated dissolved stock solution be stored for?

    1 answer

    1. The stability of Fluorescein under autoclave conditions has not been determined. Solutions have typically been filter sterilized. Long term solution stability has also not been evaluated. However, solutions of Fluorescein Diacetate are stable at -20°C in ethanol when protected from light. Similar stability would be expected with the sodium salt form.

      Helpful?

  3. Hello, I am interested in Fluorescein or Fluoresein sodium salt to perform angiography in the living mouse and would like to know if your product has a pharmacological grade. Which product is used for angiography?

    1 answer

    1. Unfortunately, a pharmacological grade Fluorescein or Fluorescein sodium salt is not available at this time.

      Helpful?

  4. Is F6377 soluble in ethanol?

    1 answer

    1. The substance is soluble in water (500 g/l) at 20°C and slightly soluble in ethanol, but insoluble in benzene, chloroform, and ether.

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service