70196

Wort Agar

suitable for microbiology, NutriSelect^® Basic

• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 17.0 g/L ; ammonium chloride, 1.0 g/L ; dextrin, 2.5 g/L ; dipotassium hydrogen phosphate, 1.0 g/L ; malt extract, 15.0 g/L ; D-maltose, 12.5 g/L ; peptone (from casein), 1.0 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 4.8±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: selective by low pH for Candida spp.; selective by low pH for Pichia spp.; selective by low pH for Saccharomyces spp.; selective by low pH for Zygosaccharomyces spp.; selective by low pH for molds (General Media); selective by low pH for yeasts (General Media)

Description

General description

Wort Agar is a general purpose mycological medium especially suitable for the cultivation and enumeration of yeasts.

Application

General purpose mycological medium especially suitable for the cultivation and enumeration of yeasts. Addition of special indicator dyes allows differentiation between yeast and bacterial colonies (Rapp).

Wort Agar has been used to cultivate Aspergillus niger.

Preparation Note

Suspend 50 g in 1 litre of distilled water. Add 2.35 g glycerol Cat. No. 49767) and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Do not overheat! Prolonged heating will diminish the gel strength of the medium.

Other Notes

Impedimetric detection of yeast and mold

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves