MABN666

Anti-TRIP8b Antibody, clone N212A/34

clone N212A/34, from mouse

• Synonym(s): PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, TPR-containing Rab8b-interacting protein, Tetratricopeptide repeat-containing Rab8b-interacting protein, Pex5Rp, TRIP8b
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: N212A/34, monoclonal
• species reactivity: human, rat, mouse
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_775175
• UniProt accession no.: Q925N3
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: rat ... Pex5L(286937)

Description

General description

Tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b) is also called PEX5-related protein, PEX5-like protein and Peroxin-5-related protein (Pex5Rp). TRIP8b a hyperpolarization-activated cyclic nucleotide-gated (HCN) channel subunit that is involved in HCN cynclic nucleotide dependence and expression at the cell surface. TRIP8b is expressed specifically in the brain.

Specificity

This antibody demonstrates specificity for TRIP8b containing exon 1b. It does not cross react with TRIP8b containing exons 1a, 2, or 4.

Immunogen

Recombinant protein corresponding to rat TRIP8b.

Application

Anti-TRIP8b Antibody, clone N212A/34 is a highly specific mouse monoclonal antibody & that targets Tetratricopeptide repeat-containing Rab8b-interacting protein & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Western Blotting Analysis: 0.5 μg/mL of this antibody detected TRIP8b in 10 μg of human brain tissue lysate.

Western Blotting Analysis: A representative lot detected TRIP8b in COS cell lysates transiently transfected with GFP tagged TRIP8b (exon1b) and not in COS cell lysates transiently transfected with TRIP8b (exon1a, 2, or 4) or with untagged Kv2.1 plasmid (Prof. J. Trimmer, University of California, Davis.).

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analyis: 0.5 μg/mL of this antibody detected TRIP8b in 10 μg of rat brain tissue lysate.

Target description

~70 kDa observed. Multiple isoforms may be observed due to alternative splicing (Lewis, A. S., et al. (2009). J Neurosci. 29(19):6250-6265.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable