MAB302
Anti-Glutamine Synthetase Antibody, clone GS-6
clone GS-6, Chemicon®, from mouse
Synonym(s):
Anti-Glutamine
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
GS-6, monoclonal
species reactivity
human, rat, mouse, sheep
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GLUL(2752)
General description
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
Specificity
Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
Immunogen
Glutamine synthetase purified from sheep brain.
Application
Detect Glutamine Synthetase using this Anti-Glutamine Synthetase Antibody, clone GS-6 validated for use in ELISA, IH, IH(P) & WB with more than 45 product citations.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neurofilament & Neuron Metabolism
Western blot: 1:1,000-1:5,000
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Target description
45 kDa
Physical form
Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Analysis Note
Control
Rat brain tissue, rat brain cytosolic fraction extract
Rat brain tissue, rat brain cytosolic fraction extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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N Hakuba et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(23), 8750-8753 (2000-01-11)
Acoustic overstimulation is one of the major causes of hearing loss. Glutamate is the most likely candidate neurotransmitter for afferent synapses in the peripheral auditory system, so it was proposed that glutamate excitotoxicity may be involved in noise trauma. However
H Ueda et al.
Investigative ophthalmology & visual science, 41(12), 3908-3914 (2000-10-29)
Dystrobrevin is a newly discovered dystrophin-associated protein with multiple sites for phosphorylation on tyrosine residues. In the present study, the cellular distribution and subcellular localization of dystrobrevin were examined in the adult rat retina, cornea, lens, iris, ciliary body, and
Alterations in protein expression and membrane properties during Muller cell gliosis in a murine model of transient retinal ischemia.
Hirrlinger PG, Ulbricht E, Iandiev I, Reichenbach A, Pannicke T
Neuroscience Letters null
Markel Olabarria et al.
Molecular neurodegeneration, 6, 55-55 (2011-08-02)
Astrocytes are fundamental for brain homeostasis and the progression and outcome of many neuropathologies including Alzheimer's disease (AD). In the triple transgenic mouse model of AD (3xTg-AD) generalised hippocampal astroglia atrophy precedes a restricted and specific β-amyloid (Aβ) plaque-related astrogliosis.
Takahiko Matsuda et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(1), 16-22 (2003-11-07)
The large number of candidate genes made available by comprehensive genome analysis requires that relatively rapid techniques for the study of function be developed. Here, we report a rapid and convenient electroporation method for both gain- and loss-of-function studies in
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