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AG100

Sigma-Aldrich

Human IgG Fc Control Protein, recombinant protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42

biological source

human

Quality Level

Assay

>90% (Coomassie blue staining, SDS-PAGE)

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

shipped in

dry ice

General description

Product Source: Mammalian cells, HEK293 cell line
Recombinatant fusion proteins consisting of the extracellular domain of immunoregulatory proteins and the constant (Fc) domain of immunoglobulin G (IgG) represent a growing class of human therapeutics. Chimeric protein of Human target protein and Human IgG-Fc domain can be purified using protein A chromatography.
This product is the control protein, Human IgG-Fc domain, purified using protein A-agarose (Millipore Corp, cat. # 16-125). MW ~ 55 kDa.

Application

Research Category
All
This product should be used as the control protein in the activity test of chimeric protein of Human target protein and Human IgG-Fc domain.

Other applications of Human IgG-Fc domain.

Packaging

Active, as purified using protein A-agarose (Millipore Corp, cat. # 16-125).

Physical form

50µg of recombinant protein in 50µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.

Storage and Stability

Shipped on dry ice. Best stored at -80 C for up to 6 months after date of receipt. Thawed aliquots should be used immediately, as repeated freeze/thaw cycles might result in loss of activity.

Analysis Note

Western blotting by primary antibody Mouse anti-Human IgG, clone HP6017, Fc (Millipore Corp, cat.# MAB1302) and secondary antibody Goat anti-Mouse IgG, HRP conjugate (Millipore Corp, cat.# 12-349).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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