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16-246

Sigma-Aldrich

Anti-EGFR Antibody, neutralizing, clone LA1, Alexa Fluor™ 488

clone LA1, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LA1, monoclonal

species reactivity

human, mouse, rat

manufacturer/tradename

Upstate®

technique(s)

immunofluorescence: suitable

isotype

IgG1

NCBI accession no.

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

UniProt accession no.

P00533

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

General description

Included Negative Control: Catalog # 16-240, Alexa Fluor™ 488-conjugated Normal Mouse IgG.

Inhibits A431 cell growth. Recognizes an antigenic determinant located on the extracellular domain of the receptor.

Specificity

external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors.

Immunogen

Human A431 membrane proteins

Application

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-EGFR Antibody, neutralizing, clone LA1, Alexa Fluor 488 is validated for use in IF for the detection of EGFR.

Quality

Routinely evaluated by positive immunostaining for the EGF receptor in A431 cells.

Storage and Stability

1 year at 4°C from date of shipment

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matthew J Crow et al.
AJR. American journal of roentgenology, 192(4), 1021-1028 (2009-03-24)
The goal of this study was to assess whether immunolabeled nanoparticle biomarkers are comparable to fluorescent marker imaging in measuring epidermal growth factor receptor (EGFR) expression. EGFR expression was quantified using both imaging methods in four cell lines: A431 human
Epidermal growth factor inhibition in solid tumours.
Ganti, Apar Kishor and Potti, Anil
Expert opinion on biological therapy, 5, 1165-1174 (2005)
Matthew J Crow et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 79(1), 57-65 (2010-12-25)
Fluorescence-based flow cytometry measures multiple cellular characteristics, including levels of receptor expression, by assessing the fluorescence intensity from a population of cells whose cell surface receptors are bound by a fluorescently labeled antibody or ligand for that receptor. Functionalized noble
Structure and function of the epidermal growth factor (EGF/ErbB) family of receptors.
Leahy, Daniel J
Advances in Protein Chemistry, 68, 1-27 (2004)
Cetuximab.
Goldberg, Richard M
Nature Reviews. Drug Discovery, Suppl, S10-S11 (2005)
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