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Key Documents

06-1328

Sigma-Aldrich

Anti-Themis Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

thymocyte selection associated, thymocyte selection pathway associated, chromosome 6 open reading frame 190, chromosome 6 open reading frame 207, protein THEMIS, Thymocyte-expressed molecule involved in selection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

rat (73% sequence homology)

technique(s)

flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_848781

UniProt accession no.

Q8BGW0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Themis(210757)

Related Categories

General description

Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.

Specificity

This antibody recognizes Themis at the C-terminus.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.

Application

Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.

Quality

Evaluated by Western Blot in mouse thymocyte tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.

Target description

~72 kDa observed.
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa

Analysis Note

Control
Mouse thymocyte tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Themis sets the signal threshold for positive and negative selection in T-cell development.
Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster et al.
Nature null
Namrata Gautam et al.
Life science alliance, 6(12) (2023-09-23)
Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model.
Mukul Prasad et al.
Cellular & molecular immunology, 18(9), 2249-2261 (2020-11-13)
Themis is a T cell lineage-specific molecule that is involved in TCR signal transduction. The effects of germline Themis deletion on peripheral CD4+ T cell function have not been described before. In this study, we found that Themis-deficient CD4+ T
Joanna Brzostek et al.
Nature immunology, 21(2), 186-198 (2020-01-15)
T cell homeostasis and functional responsiveness require signals from self-peptide-major histocompatibility complex (self-pMHC) and cytokines, but the mechanisms controlling this signal integration are unknown. Using a conditional deletion of the T cell lineage-specific protein Themis, we show that Themis is
Differential function of Themis CABIT domains during T cell development.
Okada, T; Nitta, T; Kaji, K; Takashima, A; Oda, H; Tamehiro, N; Goto, M; Okamura et al.
Testing null

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