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U3635

Sigma-Aldrich

Universal Antibody Dilution Buffer

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47
Pricing and availability is not currently available.

pH

7.3-7.5

Quality Level

application(s)

hematology
histology

storage temp.

room temp

General description

Ready to use buffer for dilution of antibodies in all immunoassays. This buffer does not contain any mammalian proteins, phosphate, sodium azide or mercury preservatives and can be used for dilution of all antibodies, including peroxidase conjugates and antibodies to phosphoproteins. Not suitable for dilution of antibodies to S100 proteins.

Application

For dilution of primary or secondary antibodies. The diluted antibodies can be stored at 2-8 °C for several weeks in this buffer.
Universal Antibody Dilution Buffer has been used in immunoblotting.[1]

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XIV (Authorisation List)

CAS No.

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Certificates of Analysis (COA)

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Zhihui Li et al.
Cell biology international, 46(10), 1672-1681 (2022-07-14)
Bladder cancer (BC) is a common urological malignancy that still lacks an effective treatment. Doxorubicin (Dox) has been widely used in the treatment of various cancers, including BC. However, chemoresistance often hampers the clinical application of Dox, therefore, it is
High level of calpain1 promotes cancer cell invasion and migration in oral squamous cell carcinoma.
Ma D, et al.
Oncology Letters, 13(6), 4017-4026 (2017)
Caitlyn Fastenau et al.
Brain pathology (Zurich, Switzerland), 34(4), e13267-e13267 (2024-05-10)
Glycosylation is the most common form of post-translational modification in the brain. Aberrant glycosylation has been observed in cerebrospinal fluid and brain tissue of Alzheimer's disease (AD) cases, including dysregulation of terminal sialic acid (SA) modifications. While alterations in sialylation
Caitlyn Fastenau et al.
GeroScience, 45(3), 1539-1555 (2023-03-04)
Terminal sialic acid residues are present on most glycoproteins and glycolipids, but levels of sialylation are known to change in the brain throughout the lifespan as well as during disease. Sialic acids are important for numerous cellular processes including cell
Da Ma et al.
Oncology letters, 13(6), 4017-4026 (2017-06-08)
Calpain1, termed conventional calpain and a member of the Ca2+-dependent neutral cysteine proteases, is considered to be involved in cancer formation and development. However, the effect of calpain1 on oral squamous cell carcinoma (OSCC) remains poorly understood. The aim of

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. How are the Universal Antibody Dilution Buffers, Products U3635 and U3510, to be used?

    1 answer
    1. The dilution buffer can be used with either primary or secondary antibodies. It is "ready-to-use"; that is, the dilution buffer itself does not need to be further diluted before use.Dilute the antibody as recommended in the antibody manufacturer's protocol. Generally, antibodies that have been diluted with Product U3635/U3510 can be stored refrigerated at 2-8°C for several weeks.

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