Skip to Content
Merck
All Photos(1)

Documents

TT0010

Sigma-Aldrich

Trypsin Spin Columns

for proteomics

Synonym(s):

Immobilized TPCK- treated Trypsin, Immobilized Trypsin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

grade

for proteomics

Quality Level

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Enjoy all of the advantages of Proteomics Grade Trypsin in a 15-minute digestion. A staple of MS sample preparation, efficient tryptic digestion is essential to successful proteomic analyses. While traditional digests require up to 18 hours, the same digest can be accomplished in only 15 minutes using the Trypsin Spin Column, Proteomics Grade.

This ultra-micro spin column contains highly purified, TPCK-treated porcine trypsin immobilized on a spherical 20 micron silica support, chemically modified to minimize non-specific adsorption. This product is ideal for rapid protein digestion of small volumes (100 μl or less). Eluted peptides are ready for MS analysis, and require no additional clean-up.

Features and Benefits

Discover the advantages for yourself!
  • 15 minute digestion
  • Ideal for 10 to 100 μg of protein sample
  • Minimal chymotryptic activity
  • No additional clean-up required

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Julianne Backiel et al.
Biochemistry, 47(43), 11273-11284 (2008-10-04)
Enzymes of the Rnf family are believed to be bacterial redox-driven ion pumps, coupling an oxidoreduction process to the translocation of Na+ across the cell membrane. Here we show for the first time that Rnf is a flavoprotein, with FMN
Hans W Hahn et al.
Journal of proteome research, 8(9), 4225-4230 (2009-07-31)
Trypsin was immobilized on glycidylmethacrylate-co-divinylbenzene (GMA/DVB) polymerized in pipet tips for online enzymatic digestion of proteins. The major advantages of in-tip digestion are easy handling and small sample amount required for analysis. Microwave-assisted digestion was applied for highly efficient and
Tomoharu Takeuchi et al.
Biological & pharmaceutical bulletin, 34(7), 1139-1142 (2011-07-02)
To study the endogenous counterpart of LEC-6, a major galectin in Caenorhabditis elegans, the proteomic analysis of glycoproteins captured by an immobilized LEC-6 column was performed using the nano liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. A protein recovered in a
Jiangjiang Liu et al.
Journal of the American Society for Mass Spectrometry, 20(5), 819-828 (2009-02-10)
This work uses electrospray ionization mass spectrometry (ESI-MS) in conjunction with hydrogen/deuterium exchange (HDX) and optical spectroscopy for characterizing the solution-phase properties of cytochrome c (cyt c) after heat exposure. Previous work demonstrated that heating results in irreversible denaturation for
Shama P Mirza et al.
Journal of proteome research, 7(7), 3042-3048 (2008-05-31)
Proteomics-based quantification methods for differential protein expression measurements are among the most important and challenging techniques in the field of mass spectrometry. Though numerous quantification methods have been established, no method meets all the demands for measuring accurate protein expression

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service