P1034

Phenyl isothiocyanate

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

• Synonym(s): PITC
• Linear Formula: C₆H₅NCS
• CAS Number: 103-72-0
• Molecular Weight: 135.19
• Beilstein: 471392
• EC Number: 203-138-1
• MDL number: MFCD00004798
• UNSPSC Code: 12352118
• PubChem Substance ID: 24898144
• NACRES: NA.31

Properties

• grade: Sigma Grade
• Quality Level: 100
• Assay: ≥99% (GC)
• form: liquid
• concentration: 8.36 M
• refractive index: n20/D 1.6515 (lit.)
• bp: 218 °C (lit.)
• mp: −21 °C (lit.)
• density: 1.132 g/mL at 20 °C (lit.)
• suitability: suitable for solid phase protein sequencing analysis
• storage temp.: 2-8°C
• SMILES string: S=C=Nc1ccccc1
• InChI: 1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H
• InChI key: QKFJKGMPGYROCL-UHFFFAOYSA-N

Description

General description

Phenyl isothiocyanate belongs to the class of aromatic compounds with isothiocyanate groups. It is typically used as a precolumn derivatization agent for the formation of phenylthiocarbamyl amino acids for their subsequent determination using chromatography techniques.

Application

Phenyl isothiocyanate may be used in the polypeptide and protein sequencing at the subpicomole and subnanomole levels, respectively, by applying automated Edman chemistry followed by the analysis using high-performance liquid chromatography (HPLC) technique.

Derivatizing reagent for primary and secondary amines. Used in sequencing peptides by Edman degradation and in amino acid analyses by HPLC.

Packaging

One mL size packaged in flame sealed amber ampules under argon.
Ten mL size packaged in screw cap amber vials under argon.

Safety Information

• Pictograms: GHS06,GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H301,H314,H317,H334
• Precautionary Statements: P261 - P272 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 190.4 °F - closed cup
• Flash Point(C): 88 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves