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F1051

Sigma-Aldrich

Fetal Bovine Serum

Canada origin, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤25 mg/dL

origin

Canada origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems. It also protects cells from oxidative damage and apoptosis.
Fetal bovine serum is the main component present in almost all media used for the culture of animal cells. It provides serum additives to the cell culture medium, which is essential for supporting growth of a variety of cell types. It is rich in growth factors and protein but low in γ-globulin content.

Application

FBS sourced from Canada is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture. It was used as a supplement for different cell cultures.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Irza Sukmana et al.
Journal of biomedical materials research. Part A, 92(4), 1587-1597 (2009-05-14)
We describe an in vitro culture process that uses 100-microm diameter poly(ethylene terephthalate) monofilaments as contact guidance of human umbilical vein endothelial cells (HUVECs) to orient the development of microvessels in a 3D environment. Untreated fibers, distanced either by 0.05
Megha S Even et al.
Trends in biotechnology, 24(3), 105-108 (2006-01-24)
Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice
Thomas Sanderson et al.
European journal of pharmacology, 593(1-3), 92-98 (2008-07-22)
Certain lactone-containing secondary plant metabolites display potent biological effects, including anti-tumor activities. This is of particular interest as these compounds appear effective against hormone-dependent cancers, such as those of breast and prostate, of which the incidence is on the rise.
Irza Sukmana et al.
Biomaterials, 31(19), 5091-5099 (2010-03-30)
In the present study, polymer monofilaments were embedded in fibrin seeded with human umbilical vein endothelial cells (HUVEC) to guide HUVEC attachment and migration in order to form oriented vessel-like structures between adjacent monofilaments. Histology and fluorescent fibrin experiments confirmed
Christopher R Hansebout et al.
Neural regeneration research, 7(28), 2165-2175 (2012-10-05)
Previous studies have shown that transplanted enteric glia enhance axonal regeneration, reduce tissue damage, and promote functional recovery following spinal cord injury. However, the mechanisms by which enteric glia mediate these beneficial effects are unknown. Neurotrophic factors can promote neuronal
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