β-Estradiol 6-((O-carboxy-methyl)oxime: BSA, Product Number E5630, is soluble in PBS to 0.90-1.10 mg/mL, and this will yield a faintly hazy to hazy, colorless to faintly yellow solution. Accordingly, E5630-5MG is soluble in 4.5-5.6 mL of PBS. The actual batch-specific solubility is listed on the corresponding CoA. Please download the CoA from the “Documents” section of the PDP at the following link:
https://www.sigmaaldrich.com/product/sigma/e5630#product-documentation
Pricing and availability is not currently available.
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sterility
non-sterile
Quality Level
form
lyophilized powder
extent of labeling
~30 mol steroid per mol BSA
solubility
phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
General description
Application
β-Estradiol 6-(O-carboxymethyl)oxime: BSA has been used:
- to test its effect on the N-methyl-D-aspartate receptor (NMDAR) currents in female dorsal root ganglion (DRG) neurons[2]
- to coat microplates for the detection of plasma 17β-Estradiol levels using indirect enzyme-linked immunosorbent assay (ELISA)[3]
- to test its effect on MutL homolog 1 in colorectal cancer lines[4]
Biochem/physiol Actions
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Certificates of Analysis (COA)
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Buongiorno, il datasheet incluso con il prodotto E56-30 non è chiaro. Potrei sapere il volume finale in cui sciogliere 5mg di E2/BSA e la relativa concentrazione?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
We have solubilized the product in phosphate buffered saline at 1 mg/mL. Solutions may be somewhat hazy.
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How is Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA, prepared?
1 answer-
Our method of preparation is considered proprietary information However, a method of preparation (not necessarily Sigma-Aldrich’s) has been described in J. Biol. Chem., 228, 713-727 (1957).
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What is the solution stability of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
We have not performed solution stability studies, and have found no information on solution stability in the literature. We would therefore recommend using freshly prepared solutions.
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What is the molecular weight of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
The Certificate of Analysis has lot specific information for the number of moles of steroid per mole of bovine serum albumin. From this data and the molecular weights of the components, an approximate molecular weight for the compound can be calculated. For example, if the moles of beta estradiol to BSA is 32, then the approximate molecular weight for the compound is 76.4 kDa (BSA has a molecular weight of approximately 66.4 kDa and 32 moles of estradiol is approximately 10 kDa more).
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What is the shelf life of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
There is no assigned shelf life date for the product. The guarantee of quality is one year from the time customer receives the product for unopened bottles stored at 2-8°C.
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