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C7902

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

Linear Formula:
CaCl2 · 2H2O
CAS Number:
10035-04-8
Molecular Weight:
147.01
NACRES:
NA.71
PubChem Substance ID:
24892994
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
MFCD00149613
Assay:
≥99.0%
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
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product line

BioReagent

Quality Segment

assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H319

Hazard Classifications

Eye Irrit. 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

Storage Class

11 - Combustible Solids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10035-04-8

CAS No.

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