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87961

Sigma-Aldrich

Tetranitroblue tetrazolium chloride

≥85% (AT)

Synonym(s):

3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], TNBT, Tetranitrotetrazolium blue chloride

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About This Item

Empirical Formula (Hill Notation):
C40H28Cl2N12O10
CAS Number:
1184-43-6
Molecular Weight:
907.63
Beilstein:
3897475
EC Number:
214-665-1
MDL number:
MFCD00036338
UNSPSC Code:
12352204
PubChem Substance ID:
57653280
NACRES:
NA.83

Assay

≥85% (AT)

form

powder or crystals

ign. residue

≤0.1%

loss

≤25% loss on drying

mp

~170 °C (dec.)

solubility

2-methoxyethanol: 0.090 g/10 mL (hot)

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccc(cc4)[N+]([O-])=O)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccc(cc8)[N+]([O-])=O

InChI

1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2

InChI key

VCESGVLABVSDRO-UHFFFAOYSA-L

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General description

crystallized with ~2 mol ethanol

Other Notes

Review: Tetrazolium salts and formazans; Detection of hydrolases (with indoxyl substrates)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Precautionary Statements

P201 - P308 + P313

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Nitrotetrazolium Blue chloride ≥90.0% (HPLC)

Sigma-Aldrich

N6876

Nitrotetrazolium Blue chloride

F.P. Altman
Progress in Histochemistry and Cytochemistry, 9, 51-51 (1976)
R Gossrau
Histochemistry, 58(3), 203-218 (1978-12-01)
Using freeze-dried or sections from fresh-frozen or aldehyde-fixed material nitro BT (NBT), tetranito BT (TNBT), distyryl nitro BT (DS-NBT), thiocarbamyl nitro BT (TC-NBT) or benzothiazolylstyrylphthalhydrazidyl tetrazolium chloride (BSPT) were tested as auxiliary reagents for the localization of glycosidases, phosphatases and
R L Gardner
Journal of embryology and experimental morphology, 80, 251-288 (1984-04-01)
Conditions were found for staining whole mid-gestation capsular parietal endoderms and visceral yolk sacs for malic enzyme activity that gave excellent discrimination between wild-type (Mod-1+/Mod-1+) cells and mutant (Mod-1n/Mod-1n) cells that lack the cytoplasmic form of the enzyme. Reciprocal blastocyst
C L Jordan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(1), 239-247 (1989-01-01)
In the previous study (Jordan et al., 1989), we demonstrated that androgen treatment of juvenile male rats inhibits the elimination of synapses in the levator ani (LA) muscle. In the present study, we asked whether synapse elimination would occur once
W M Frederiks et al.
The Histochemical journal, 25(1), 86-91 (1993-01-01)
A quantitative histochemical procedure was developed for the demonstration of purine nucleoside phosphorylase in rat liver using unfixed cryostat sections and the auxiliary enzyme xanthine oxidase. The optimum incubation medium contained 18% (w/v) poly(vinyl alcohol), 100 mM phosphate buffer, pH
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