73144

Resorufin

95%

• Synonym(s): 7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
• Empirical Formula (Hill Notation): C₁₂H₇NO₃
• CAS Number: 635-78-9
• Molecular Weight: 213.19
• Beilstein: 174850
• EC Number: 211-241-8
• MDL number: MFCD00128991
• UNSPSC Code: 12171500
• PubChem Substance ID: 329764655
• NACRES: NA.32

Properties

• Assay: ≥95.0% (HPLC); 95%
• form: solid
• mp: >300 °C (lit.)
• storage temp.: −20°C
• SMILES string: O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
• InChI: 1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
• InChI key: HSSLDCABUXLXKM-UHFFFAOYSA-N

Description

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.

Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H₂O₂ by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable