Journal of proteome research, 11(8), 4269-4276 (2012-07-10)
In large-scale phosphoproteomics studies, fractionation by strong cation exchange (SCX) or electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) is commonly used to reduce sample complexity, fractionate phosphopeptides from their unmodified counterparts, and increase the dynamic range for phosphopeptide identification. However, these procedures
Journal of proteome research, 10(8), 3474-3483 (2011-06-21)
Reversible phosphorylations play a critical role in most biological pathways. Hence, in signaling studies great effort has been put into identification of a maximum number of phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have been proven to be an
The HIV-1 regulatory proteins tat and rev are both RNA binding proteins which recognize sequences in duplex RNA which are close to structural distortions. Here we identify phosphate contacts which are critical for each binding reaction by use of a
Journal of chromatography. A, 782(1), 55-62 (1998-01-24)
A new two-step high-performance liquid chromatography (HPLC) procedure has been developed to separate modified histone H1 subtypes. Reversed-phase (RP) HPLC followed by hydrophilic-interaction liquid chromatography (HILIC) was used for analytical and semi-preparative scale fractionation of multi-phosphorylated H1 histone subtypes into
Methylphosphonic acid (MPn) is suspected to play an important role in aquatic systems like rivers or the open ocean. To gain more insights into the importance of MPn, e.g., for the aquatic phosphorus cycle, an analytical method for its quantitative
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