198269

Erythrosin B

certified by the Biological Stain Commission, Dye content ≥85 %

• Synonym(s): Erythrosin extra bluish, 2′,4′,5′,7′-Tetraiodofluorescein disodium salt, Acid Red 51, Iodoeosin
• Empirical Formula (Hill Notation): C₂₀H₆I₄Na₂O₅
• CAS Number: 16423-68-0
• Molecular Weight: 879.86
• Colour Index Number: 45430
• Beilstein: 1443945
• EC Number: 240-474-8
• MDL number: MFCD00144257
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851871
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥85%
• color: dark red
• pH: 6.4 (30.3 °C)
• mp: 315 °C
• solubility: H₂O: 1 mg/mL, clear
• density: 0.625 — 0.731 g/cm³ at 30.1 °C (86.2 °F)
• λmax: 525 nm
• ε (extinction coefficient): ≥13000 at 308-312 nm; ≥32000 at 259-263 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]c1c(I)cc2c(Oc3c(I)c([O-])c(I)cc3C24OC(=O)c5ccccc45)c1I
• InChI: 1S/C20H8I4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2
• InChI key: RAGZEDHHTPQLAI-UHFFFAOYSA-L

Description

Application

Erythrosin B has been used as a dye in fluorescence lifetime imaging.

Biochem/physiol Actions

Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. Erythrosin B is an artificial food dye that induces hyperkinesis when swallowed by susceptible children. It plays a major role in inhibiting dopamine uptake and high affinity ³H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.

Suitability

Counterstain to alum-hematoxylin.

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H302,H411
• Precautionary Statements: P264 - P270 - P273 - P301 + P312 - P391 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): 359.6 °F - closed cup
• Flash Point(C): 182 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves