11699113001

MgCl₂ Stock Solution

pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL

• Synonym(s): magnesium chloride solution
• UNSPSC Code: 41116100

Properties

• form: solution
• Quality Level: 100
• packaging: pkg of 3 × 1 mL
• manufacturer/tradename: Roche
• concentration: 25 mM
• technique(s): PCR: suitable
• pH: 8.25-8.45 (25 °C)
• storage temp.: −20°C

Description

General description

The MgCl₂ Stock Solution is used for the individual adjustment of the Mg²⁺-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl₂. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and the yield of the PCR reaction.

Application

The combination of PCR Buffer without MgCl₂ and the MgCl₂ Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl₂ Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).

Biochem/physiol Actions

Magnesium (Mg²⁺) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR). It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.

Physical form

The MgCl₂ Stock Solution contains 25mM MgCl₂ in sterile water.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash