MAB331-C
Anti-SNAP-25 Antibody, clone SP14 (Ascites Free)
clone SP14, from mouse
Synonym(s):
Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP-25
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SP14, monoclonal
species reactivity
monkey, bovine, mouse, rat, guinea pig, human
technique(s)
activity assay: suitable
electron microscopy: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... SNAP25(6616)
General description
Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.
Specificity
Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.
Immunogen
Epitope: N-terminal half
Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).
Application
Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.
Target description
~25 kDa observed
Linkage
Replaces: MAB331
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Calpain activity contributes to the control of SNAP-25 levels in neurons.
Grumelli, C; Berghuis, P; Pozzi, D; Caleo, M; Antonucci, F; Bonanno, G; Carmignoto et al.
Molecular and Cellular Neurosciences null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null
Autoregulation in PC12 cells via P2Y receptors: Evidence for non-exocytotic nucleotide release from neuroendocrine cells.
Hussl, S; Kubista, H; Boehm, S
Purinergic Signaling null
Fatal familial insomnia with an unusual prion protein deposition pattern: an autopsy report with an experimental transmission study.
Sasaki, K; Doh-ura, K; Wakisaka, Y; Tomoda, H; Iwaki, T
Neuropathology and Applied Neurobiology null
Taste cells with synapses in rat circumvallate papillae display SNAP-25-like immunoreactivity.
Yang, R; Crowley, HH; Rock, ME; Kinnamon, JC
The Journal of Comparative Neurology null
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